Intended Use

“MagQu” Phospho-α-Synuclein IMR Reagent is used to quantitatively measure phosphorylated α-synuclein (p-ASC129) in human fluid specimen, such as plasma, serum or CSF.

Use “MagQu” Phospho-α-Synuclein IMR Reagent only with the XacPro-S System (MagQu Co., Ltd.). 

Introduction

Parkinson’s disease (PD) is characterized by the intraneuronal α-synuclein inclusions called Lewy bodies. Increase of phosphorylation of α-synuclein in Serine 129 (p-ASC129) has been correlated with the aggregation, toxicity, protein interaction and turnover of α-synuclein. Thus, p-ASC129 can indicate the pathogenesis of PD.^1,2

Principles of Test

“MagQu” Phospho-α-synuclein IMR Reagent is designed for rapid quantifying phosphorylated α-synuclein (p-ASC129) by ImmunoMagnetic Reduction (IMR). We conjugate antibody on the surface of around 50 nm-in-diameter Fe₃O₄ magnetic particles. When the antibodies on the surface bind with phosphorylated α-synuclein (p-ASC129), the magnetic particles form clusters. Therefore, the ac susceptibility (Xac) of magnetic particles would be reduced in the adding ac magnetic field. By measuring the reduction of Xac, phosphorylated α-synuclein (p-ASC129) can be easily, rapidly and accurately quantified.³

References

1. Wang, Y., Shi, M., Chung, K. A., Zabetian, C. P., Leverenz, J. B., Berg, D., Srulijes, K., Trojanowski, J. Q., Lee, V. M., Siderowf, A. D., Hurtig, H., Litvan, I., Schiess, M. C., Peskind, E. R., Masuda, M., Hasegawa, M., Lin, X., Pan, C., Galasko, D., Goldstein, D. S., Jensen, P. H., Yang, H., Cain, K. C., Zhang, J. (2012). Phosphorylated α-synuclein in Parkinson's disease. Science translational medicine, 4(121), 121ra20.

2. Oueslati A. (2016). Implication of Alpha-Synuclein Phosphorylation at S129 in Synucleinopathies: What Have We Learned in the Last Decade?. Journal of Parkinson's disease, 6(1), 39-51.

3.C.C. Yang, S.Y. Yang, C. S. Ho, et al, ”Development of antibody functionalized magnetic nanoparticles for the immunoassay of carcinoembryonic antigen: a feasibility study for clinical use.”, Journal of Nanobiotechnology, 2014, 12:44.