Intended Use

“MagQu” Phosphorylated Tau Protein [pT181] Control Solution is used as a standard control for “MagQu” Phosphorylated Tau Protein [pT181] IMR Reagent (MF-PT1-0060).

Introduction

Normal phosphorylated tau is essential to neuronal homeostasis.However, hyperphosphorylation of tau induces the protein to detach from the microtubules, thereby destabilizing microtubules and affecting its axonal transport. Previous reports showed that hyperphosphorylated tau aggregates to from insoluble neurofibrillary tangles that interfere with fundamental cell functions. It has been classically associated with many neurodegenerative disease including Alzheimer disease, Parkinson's disease, Frontotemporal dementia and Lewy body disorders. “MagQu” Phosphorylated Tau Protein [pT181] IMR Reagent is an in vitro diagnostic device intended for the quantitative determination of p-tau 181 levels to help diagnosis neurodegenerative disease from human fluid specimen, such as plasma, by using the Magnetic Immunoassay Analyzer (XacPro-S).¹

Principle of Test

“MagQu” Phosphorylated Tau Protein [pT181] Control Solution is used as a standard control for“MagQu” Phosphorylated Tau Protein [pT181] IMR Reagent (MF-PT1-0060). The antibody conjugated on the surface of Fe₃O₄ magnetic particles (~ 50 nm) could bind with Tau protein and form clusters. With the increase of cluster, the signal of ac susceptibility ( χac ) will decrease in the applied ac magnetic field. That is the principle of Immuno Magnetic Reduction (IMR). By measuring the reduction of χac , we can quantify the Tau protein in the sample easily and accurately.²

References

1. Mielke MM, Hagen CE, Xu J, Chai X, Vemuri P, Lowe VJ, Airey DC, Knopman DS, Roberts RO, Machulda MM, Jack CR Jr, Petersen RC, Dage JL, “Plasma phospho-tau181 increases with Alzheimer's disease clinical severity and is associated with tau- and amyloid-positron emission tomography”, Alzheimers Dement. 1-9, (2018)
2. Che-Chuan Yang, Shieh-Yueh Yang, Jen-Jie Chieh, Herng-Er Horng, Chin-Yih Hong, Hong-Chang Yang, K. H. Chen, B. Y. Shih, Ta-Fu Chen, and Ming-Jang Chiu, “Biofunctionalized magnetic nanoparticles for specifically detecting biomarkers of Alzheimer’s disease in vitro”, ACS Chem. Neurosci. 2, 500 (2011).