Intended Use

“MagQu” Neurofilament light Control Solution is used as a standard control for “MagQu” Neurofilament light IMR Reagent (MF-NFL-0060).

Introduction

Neurofilament light(NfL) is a specific cytoskeletal protein highly expressed in largely myelinated axons. Increased levels of NfL in CSF and blood are clinically useful biomarkers in many neurodegenerative diseases including Alzheimer’s disease (AD), Parkinson’s disease (PD), Huntington disease (HD). ^1,2

Principle of Test

Neurofilament light Control Solution is used as a standard control for “MagQu” Neurofilament light IMR Reagent (MF-NFL-0060). The antibody conjugated on the surface of Fe₃O₄ magnetic particles (~ 50 nm) could bind with neurofilament light and form clusters. With the increase of cluster, the signal of ac susceptibility ( χac ) will decrease in the applied ac magnetic field. That is the principle of Immuno Magnetic Reduction (IMR). By measuring the reduction of χac, we can quantify the neurofilament light in the sample easily and accurately.³

References

1. Lewczuk, P., Ermann, N., Andreasson, U., Schultheis, C., Podhorna, J., Spitzer, P., Maler, J. M., Kornhuber, J., Blennow, K., Zetterberg, H. (2018). Plasma neurofilament light as a potential biomarker of neurodegeneration in Alzheimer's disease. Alzheimer's research & therapy, 10(1), 71. doi:10.1186/s13195-018-0404-9
2. Hansson, O., Janelidze, S., Hall, S., Magdalinou, N., Lees, A. J., Andreasson, U., Norgren, N., Linder, J., Forsgren, L., Constantinescu, R., Zetterberg, H., Blennow, K., Swedish BioFINDER study (2017). Blood-based NfL: A biomarker for differential diagnosis of parkinsonian disorder. Neurology, 88(10), 930-937. 
3. Che-Chuan Yang, Shieh-Yueh Yang, Jen-Jie Chieh, Herng-Er Horng, Chin-Yih Hong, Hong-Chang Yang, K. H. Chen, B. Y. Shih, Ta-Fu Chen, and Ming-Jang Chiu, “Biofunctionalized magnetic nanoparticles for specifically detecting biomarkers of Alzheimer’s disease in vitro”, ACS Chem. Neurosci. 2, 500 (2011).